The Genomics Core Facility offers a wide range of services, combining guidance and access to state-of-the-art technologies in the field of genomics. These services range from sample preparation, to basic sequencing, to the generation of large-scale NGS datasets.
The core facility offers complete real time PCR service and Digital Droplet PCR (ddPCR) service, with support from the RNA extraction to the final results and works closely with the IRIC’s Bioinformatics Core Facility.
Services offered:
- Capillary sequencing
- DNA sequencing (plasmids, PCR products, etc.)
- Results provided in 48 hours and personalized access to sequences on a secure server
- Real time PCR (qPCR) and Digital Droplet PCR (ddPCR)
- Next generation sequencing
- RNA-Seq
- Amplicons
- Small RNAs (miRNAs)
- Targeted genomic regions (Exome sequencing)
- ChIP-seq (chromatin immunoprecipitation sequencing)
- Whole genomic DNA sequencing (complete genome)
- Single-cell sequencing
- Access to expert advice with respect to optimal sequencing strategy and experimental design
- Validation and analysis of results
- DNA and RNA analysis
- Analysis of the quality and quantity of the RNA (full report in PDF format, RIN quality index for the RNA)
Equipment
Sanger sequencing
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DNA analyzer ABI 3730
Next-generation sequencing
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Illumina NextSeq500
Illumina MiSeq
10X Genomics Chromium Controller
qPCR
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Life Technologies ViiA 7
Life Technologies QuantStudio 7 Flex
Roche LC480
RNA and DNA quality analysis
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Agilent Bioanalyzer 2100
Spectrophotometer
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NanoDrop 8000
Digital Droplet PCR
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Biorad QX200
Sonicator
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Covaris S-series
Semi-automated pipetting stations
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- Biomek FX
- EpiMotion