The Biophysics/NMR Core Facility provides a cutting-edge solution in NMR spectroscopy with particular emphasis on experiments based on the investigation of protein structure, protein-ligand interactions and routine small molecule analysis.

Services offered:

• Analysis of samples using NMR and analysis of spectra
• Access to in-house scripts to aid the analysis of NMR data
• User advice and training in:
  • Incorporation of stable isotopes and purification of proteins
  • Developing strategies for structure determination of proteins and small molecules, and investigation of protein-ligand complexes
  • Processing and interpretation of NMR spectra
  • Use of software to generate 3D structures from NMR study data
  • Automated acquisition of spectra for small molecules
    • 1D spectra of nuclei ranging from 15N to 31P, including 1H
    • 2D spectra, including COSY, NOESY, ROESY, HSQC, HMQC, HMBC, H2BC experiments
• Implementation of NMR experiences for the assignment and structure determination of proteins, including (but not restricted to):
  • Spectra for backbone assignment; HNCACB, HNCA, CBCA(CO)NH, HNCOCA, HNCO, etc.
  • Spectra for sidechain assignment; C(CO) NH-TOCSY, HC(CO)NH-TOCSY, HCCH-TOCSY, etc.
  • Spectra for production of structural restraints; 2D, 3D, 4D 15N and 13C-edited NOESY, HNHA, HNHB
• Implementation of innovative methods for expediating NMR experiments including non-linear acquisitions and SO-FAST methods
• Determination of dipolar couplings; IPAP-HSQC
• Access to spectra for investigating small molecule binding to proteins; 1D and 2D STD experiments, 2D TCS experiments, 1D T1rho experiments, 2D 1H-15N HSQC titration experiments. 3D select filter-edit experiments