Transgenesis
 PDF Brochure
 Our Transgenesis Facility generates various mouse models for use by IRIC and Université de Montréal investigators as part of their research initiatives in immunology, hematology, virology and cancer.
Housed in a 200m2 space adjacent to our Animal Facility in IRIC's Marcelle-Coutu Pavilion, the facility is also accessible to outside scientists both from academia - namely those involved in the Réseau de recherche en transgénèse du Québec (RRTQ) - and from industry, through collaborative and contract research agreements.
Overview of Services
The following transgenesis services are currently provided at IRIC:
- DNA microinjection into the pronucleus
- ES cell injection into blastocysts
- ES cell aggregation
- genotyping
- nurse cell production (MEFs)
Gene targeting and mouse embro cryopreservation as well as technical training will also be provided.
Equipment
- two inverted microscopes Nikon TE2000-U with Hoffman-type phase contrast
- two Narishige micromanipulation and microinjection systems
- two Peltier Linkam PE100-NI units
- two Primetech Pmm-150FU piezoelectric drilling systems
- a Leica MZ-12.5 fluorescence stereomicroscope with base illumination
- two Leica MZ-12.5 stereomicroscopes with base illumination
- two Leica MZ12.5 stereomicroscopes with top illumination and motorized focus drive
- two Nikon Coolpix 5400 imaging systems
- a Sutter P97 needle puller
- a Narishige EG-400 microgrinder
- a Narishige MF-900 microforge
- a Zeiss Axiovert 40 CFL inverted microscope with fluorescence attachment
- a Leica DM IL inverted microscope
- a Leica DM LS routine microscope
Pricing Consult our current rates.
Contacts
For more information on IRIC's Transgenesis Facility, please contact:
Véronique Paradis
T + 514 343.6111 ext. 1.0518
v.paradis@umontreal.ca
Sébastien Harton
T + 514 343.6111 ext. 1.0518
sebastien.harton@umontreal.ca
Eric Milot, Ph.D.
T + 514 343.6111 ext. 1.0518
ericmilot.hmr@ssss.gouv.qc.ca
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