VINCENT ARCHAMBAULT, Ph.D.
AWARDS & HONOURS
- Maud Menten Prize for Biomedical Research from the Canadian Institutes of Health Research Institute of Genetics, 2009
- Long-Term Fellowship, Human Frontier Science Program, 2005-2009
- Long-Term Fellowship, European Molecular Biology Organization (EMBO), 2004-2005
TRAINING
- Postdoctoral training with David M. Glover, Department of Genetics, University of Cambridge, 2004-2009
- Ph.D. Biological Sciences with Frederick R. Cross and Michael P. Rout, The Rockefeller University, 1999-2004
- B.Sc. in Biochemistry, Université de Montréal, 1996-1999
RESEARCH SUPPORT
- Canada Foundation for Innovation
- Canadian Institutes of Health Research
- Fonds pour la recherche en santé du Québec
- Human Frontier Science Program
After completing his undergraduate studies in Biochemistry at the Université de Montréal, Vincent Archambault undertook his Ph.D. at The Rockefeller University where he became interested in the fundamental molecular mechanisms that control cell cycle progression. Specifically, he studied how regulation of the cyclin-dependent kinase (Cdk1) by cyclins allows the coordination of cell cycle events in the budding yeast.
His interest in studying the cell cycle in the context of a metazoan model took him to the lab of David Glover in the Department of Genetics at the University of Cambridge as an EMBO fellow and subsequently as a HFSP fellow. Using the versatile Drosophila model, Dr. Archambault studied regulators of cell division, both in the context of the whole animal and in cultured cells, combining genetics, functional genomics, biochemistry, proteomics and microscopy. In particular, he investigated the functions and regulation of two mitotic kinases: Greatwall and Polo.
The human ortholog of Polo, Polo-like kinase 1 is overexpressed in many forms of cancer, has oncogenic properties, and is the target of recently developed anti-cancer drugs. Thus it is crucial to understand how these kinases are controlled and function during the cell cycle and in development. Dr. Archambault’s work will contribute to our understanding of the molecular basis of cell division, which is often deregulated in cancer.
SELECTED PUBLICATIONS
Archambault, V. & Pinson, X. Free Centrosomes: Where do they all come from?
Fly (Austin). 2010 Apr 2;4(2).
Archambault, V., Glover, D.M. Polo-like kinases: conservation and divergence in their functions
and regulation. Nature Reviews Molecular Cell Biology, 2009, Apr;10(4):265-75.
D’Avino, P.P., Archambault, V., Przewloka, M.R., Zhang, W., Laue, E.D., Glover, D.M.,
Isolation of protein complexes involved in mitosis and cytokinesis from Drosophila
cultured cells. Methods in Molecular Biology, 2009, 545:99-112.
Archambault, V., D’Avino, P.P., Deery, M.J., Lilley, K.S., Glover, D.M. Sequestration of Polo
Kinase to microtubules by phosphopriming-independent binding to Map205 is relieved by
phosphorylation at a CDK site in mitosis. Genes & Development. 2008, Oct
1;22(19):2707-20.
Archambault, V., Chen, F., Glover, D.M., A bitter PP1 fights the sweet Polo. Molecular Cell
2008, Jun 6;30(5):541-2 (preview).
Archambault, V., Glover, D.M., Yeast Polo-like kinase substrates are nailed with the right
tools. Genome Biology. 2008, Jan 30; 9:203 (review).
Archambault, V., Zhao, X., White-Cooper, H., Carpenter, A.T.C., Glover, D.M. Mutations in
Drosophila Greatwall/Scant reveal its roles in mitosis and meiosis and interdependence
with Polo kinase. PLoS Genetics. 2007, Nov 9;3(11):e200.
D'Avino, P.P., Archambault, V., Przewloka, M.R., Zhang, W., Lilley, K.S., Laue E.D., Glover
D.M. Recruitment of Polo kinase to the spindle midzone during cytokinesis requires the
Feo/Klp3A complex. PLoS ONE. 2007, Jun 27;2:e572.
Przewloka, M.R., Zhang, W., Costa, P., Archambault, V., D'Avino, P.P., Lilley, K.S., Laue, E.D.,
McAinsh, A.D., Glover, D.M. Molecular analysis of core kinetochore composition and
assembly in Drosophila melanogaster. PLoS ONE. 2007, May 30;2:e47
Ikui, A.E, Archambault, V., Drapkin, B.J., Campbell, V., Cross, F.R. Cyclin and cyclin-dependent
kinase substrate requirements for preventing rereplication reveal the need for
concomitant activation and inhibition. Genetics. 2007, Mar;175(3):1011-22.
Chen, F., Archambault, V., Kar, A., Lio', P., D'Avino, P.P., Sinka, R., Lilley, K.S., Laue, E.D.,
Deak, P., Capalbo, L., Glover, D.M., Multiple protein phosphatases are required for
mitosis in Drosophila. Current Biology. 2007, Feb 20;17(4):293-303.
Archambault V., Cell cycle: proteomics gives it a spin. Expert Review of Proteomics. 2005, Aug;
2(4):615-25 (review).
Archambault, V., Ikui, A.E., Drapkin, B.J., Cross, F.R. Disruption of mechanisms that prevent
rereplication triggers a DNA damage response. Molecular and Cellular Biology. 2005,
Aug; 25(15):6707-21.
Archambault, V., Buchler, N.E., Wilmes, G.M., Jacobson, M.D., Cross, F.R. Two-faced cyclins
with eyes on the targets. Cell Cycle. 2005, Jan 17; 4(1):125-30.
Chang, E.J., Archambault, V., McLachlin, D.T., Krutchinsky, A., Chait, B.T. Analysis of protein
phosphorylation by hypothesis-driven multiple stage mass spectrometry. Analytical
Chemistry. 2004, Aug 1;76 (15):4472-83.
Archambault, V., Chang, E., Drapkin, B.J., Cross, F.R., Chait, B.T., Rout, M.P. Targeted
proteomic study of the cyclin-Cdk module. Molecular Cell. 2004, June 18;.14(6):699-711.
Wilmes, G.M., Archambault, V., Austin, R.J., Jacobson, M.D., Bell, S.P., Cross, F.R. Interaction
of the S-phase cyclin Clb5 with an ‘RXL’ docking sequence in the initiator protein Orc6
provides an origin-localized replication control switch. Genes & Development. 2004, May
1; 18(9):981-91.
Archambault, V., Li, C.X., Tackett, A.J., Wasch, R., Chait, B.T., Rout, M.P., Cross, F.R. Genetic
and biochemical evaluation of the importance of Cdc6 in regulating mitotic exit.
Molecular Biology of the Cell. 2003, Nov; 14(11):4592-604.
Cross, F.R., Archambault, V., Miller, M.E., Klovstad, M. Testing a mathematical model of the
yeast cell cycle. Molecular Biology of the Cell. 2002, Jan 24; 13(1):52-70.
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